Filter Flask Parts
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Disclaimer: This photo is sourced from many relied on internet sites, photo copyright depends on the proprietor of the photo, we do not acknowledge this picture as our residential property or work.18.104.22.168 Place an SPE disk on the base of the filter holder (Figure 4) and wet with toluene. While holding a GMF 150 filter above the SPE disk with tweezers, wet the filter with toluene and lay the filter on the SPE disk, making sure that air is not trapped between the filter and disk. Clamp the filter and SPE disk between the 1 L glass reservoir and the vacuum filtration flask. 22.214.171.124 Rinse the sides of the filtration flask with approx 15 mL of toluene using a squeeze bottle or syringe.Wildman trap flask. Stopper on shaft is lifted up to neck of flask to trap off floating layer. plunger is held above the mass of material settling at bottom of flask. Let flask stand strictly undisturbed 1 hr more. 6. Raise stopper or disk and trap off into.beaker. 7. Add 40 ml heptane and mix in. Let stand undisturbed 1.5 hr. 8. Trap off into same beaker as above. 9. While stopper is in "trap position," rinse rod and neck of flask with 60% alcohol and add rinsings to beaker. 10. Filter onto ruled filter Vacuum Equipment SigmaAldrich® vacuum filtration assembly vacuum filtration apparatus for HPLC solvent purification, particulate analysis. Oacterio logical studies, radiochemical determinations, quantitative gravimetnc analysis, and similar applications. Clamp seals filter membrane between flanges on funnel top and funnel stem. Includes funnel top, funnel stenVmembrane support, aluminum clamp, stopper (except standard taper units), filtration flask (when indicated).Keep all.parts from each mixer head separated from those of the other s'ssemblies. Brush all parts and rinse thoroughly with tap Mark the level of the combined alcohol eluates in the 1liter filter flask and quantitatively transfer it to a 2liter separatory funnel. 11. Add water to the marked level; add 100 milliliters of water and 20 milliliters of saturated sodium sulfate solution to the flask, mix, and transfer the mixture to the separatory funnel. 12. Add 100 milliliters of chloroform and shake Keep all parts from each mixer head separated from those of the other assemblies. Brush all parts and rinse thoroughly with tap Mark the level of the combined alcohol eluates in the 1liter filter flask and quantitatively transfer it to a 2liter.separatory funnel. 11. Add water to the marked level: add 100 milliliters of water and 20 milliliters of saturated sodium sulfate solution to the flask, mix, and transfer the mixture to the separatory funnel. 12. Add 100 milliliters of chloroform and shake Suitable for detection of RNADNA complexes using 4611H62 Filter. Funnel, 60 mm long x 25 mm o.d., has capacity of 20 ml, screws into an inverted cap fitting containing a Teflonencased, fritted glass membrane support disc. Glass delivery stem is 80 mm long x 8 mm o.d., with drip tip; mounts to bottom portion of Teflon center section by threaded metal fitting. Overall length 180 mm. With size 7 onehole Neoprene stopper for mounting in filter flask, but without flask or membrane purified.by digestion with hydrochloric acid and thorough washing with water. The filter tube passes through a lholed rubber stopper which fits into a side arm filtering flask. The side arm of this flask is connected to the suction pipe by a 2way stopcock. In the top of the filter tube is fitted a 2holed rubber stopper carrying glass tubes bent at right angles. To these tubes are attached rubber tubing bearing pinchcocks. One of these rubber tubes terminates in a tube containing soda lime.purified by digestion with hydrochloric acid and thorough washing with water. The filter tube passes through a 1holed rubber stopper which fits into a side arm filtering flask. The side arm of this flask is connected to the suction pipe by a 2way.stopcock. In the top of the filter tube is fitted a 2holed rubber stopper earrying glass tubes bent at right angles. To these tubes are attached rubber tubing bearing pinchcocks. One of these rubber tubes terminates in a tube containing soda lime.Add enough 60% ethanol to the flask so that only the heptane layer is above the rubber stopper. Let stand to allow heptane layer to form at the top, trap off the heptane layer, and filter it through filter paper in a Buchner funnel. 8. Add 9 ml more heptane to solution. Mix, then let stand until heptane layer rises to the top. Trap off the heptane layer, and filter it through filter paper (i.e., new piece of filter paper; not piece used in Parts 3 and 4) in a Buchner funnel. 9. Examine the filter.paper
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